Even with extensive planning and simulations, relocating or opening a new unit can present unanticipated patient safety challenges. This study describes the quantity, rate, severity score, and category of safety events submitted in the 90 days before and after relocation of the pediatric, cardiac, and neonatal intensive care units of one hospital. There were no statistically significant changes in severity of safety events before and after relocation, and only two categories of events showed statistically significant changes in quantity of reported events.