An incident learning system (ILS) can highlight system weaknesses and promote organizational learning to prevent future harm. This study aimed to assess staff reporting patterns and perspectives on safety culture after implementing a new ILS in a radiation oncology department. The percentage of respondents who reported feeling comfortable or very comfortable reporting increased from 49% to 75% after implementation. Post-implementation, 92% of staff felt the department practiced a no-blame culture, and nearly half thought the department showed an ability to learn. The biggest barrier to reporting in both periods was the amount of time it took to submit a report.