Correctly reporting, interpreting, and comparing patient safety culture (PSC) survey findings is critical to improving patient safety in hospitals. Nearly 1,800 physicians and nurses were surveyed using the Hospital Survey on Patient Safety Culture (HSOPS) to examine the influence of gender, profession, and managerial function on perception of patient safety culture. Results indicate that profession and managerial function had significant direct effects, while gender had indirect effects, on the Overall Perception of Patient Safety dimension. Hospitals should take these characteristics into account when interpreting PSC survey results.